Abstract
Although conventional ion-exchange HPLC and ion-pair reversed phase HPLC using tetrabutylammonium hydroxide allowed ready determination of uridine 5′-diphospho-α- d-glucose and its hydrolysis products, neither method was suitable for the determination of uridine 5′-diphospho-α- d-glucuronic acid (α-UDPGA). However, mixed ion-pair reversed-phase HPLC, using a combination of tetrabutylammonium hydroxide and tetraethylammonium hydroxide (1:1), has been found to offer a convenient and effective means of simultaneously determining α-UDPGA and its hydrolysis products, UDP, UMP and cUMP.
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