Mixed-function oxidase studies in the redfish, sciaenops ocellata, from galveston bay, texas

Abstract

1. The metabolism of parathion to para-nitrophenol (PNP) in redfish ( Sciaenops ocellata) liver microsomes has been both identified and characterized. 2. This mixed-function oxidase (MFO) reaction in redfish requires NADPH and is inhibited by carbon monoxide. It exhibits a temperature optimum of 25°C but no clear pH optimum between 7.0–8.5. 3. Redfish hepatic microsomal MFOs were not induced by 2.5 days after a single i.p. injection of 88 mg Aroclor 1254/kg body wt, but under the same dosage and time conditions male albino Swiss mice were significantly induced ( p < 0.05).