Abstract
To investigate the effect of caffeic acid phenethyl ester (CAPE) on local and systemic inflammation and bone loss in collagen antibody-induced arthritis (CAIA) mice. Four groups of mice (n=8 per group) were allocated; control, CAPE (1mg/kg), CAIA and CAIA+CAPE (1mg/kg). Local inflammation and bone loss were evaluated using clinical paw scores, in vivo micro-computed tomography (micro-CT), histological assessment and tartrate-resistant acid phosphatase (TRAP) staining. Serum levels of C-reactive protein (CRP) and C-terminal telopeptide (CTX-1) were measured by ELISA. Jejunum and colon sections were evaluated histopathologically for damage and toxicity. Greater paw scores and percentage change in paw volume were observed in CAIA+CAPE compared to the control groups (p<0.05). Bone volume over time remained unchanged (p=0.94) and the number of multinucleated TRAP-positive cells was greatest in CAIA+CAPE mice (p<0.05). CRP and CTX-1 levels did not differ between groups. CAIA+CAPE mice exhibited lower colon toxicity scores and a reduced percentage of cavitated goblet cells in the colon crypts compared with CAIA mice (p=0.026 and p=0.003, respectively). Histopathology in the jejunum was not altered. CAPE did not reduce paw inflammation or bone loss in CAIA mice. CAPE reduced histopathological changes in the colon of CAIA mice.
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