Abstract
Synthesis of mixed-backbone oligonucleotides containing segments of deoxynucleoside. phosphorothioate and 2'-O-methylribonucleoside methylphosphonate have been achieved by using N-Pent-4-enoyl (PNT) heterocyclic base-protected 2'-O-methylribonucleoside methylphosphonamidites. Use of PNT for heterocyclic base-protection allows the deprotection to be carried out under milder conditions without affecting base labile methylphosphonate internucleotide linkages. New MBOs have reduced phosphorothioate internucleotide linkages while maintaining affinity with target RNA and are stable towards nucleases.
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