Abstract

Samples taken at intervals from a synchronised mitotic diploid culture of Saccharomyces cerevisiae were treated with ultraviolet light and with photoreactivating white light. They were scored for survival and for the incidence of intergenic and intragenic recombination. It was found that the frequency of all three events varied through the cycle, cell death and intergenic recombination reaching peaks during DNA synthesis and intragenic recombination at the end of DNA synthesis. The periods of maximum photoreactivation coincided with periods of maximum induction of a given event by the ultraviolet light. While cell death showed some photoreactivation at all stages of the cycle, both kinds of UV-induced recombination at some stages responded hardly or not all to photoreactivating light. The interpretation of the results is discussed.

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