Abstract
An anomalous mitosis was observed in HeLa cells within 24 hr after a shift in growth temperature from 37 °C to 29 °C. The anomalous mitosis was characterized by ( a) the presence of a mitotic spindle, ( b) the formation of a “metaphase plate” which passed through a number of diffusions and reformations prior to the onset of “anaphase”, ( c) the failure of the separation of sister chromatids in the kinetochore region, ( d) the asynchronous movement and random distribution of (undivided) chromosomes towards the poles. The induction of the anomalous mitosis appeared to be dependent upon the time spent by the interphase cell at 29 °C rather than on the specific phase of the cell cycle during which the temperature shift occurred.
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