Abstract
Cell division was arrested by injection of a preimmune rabbit serum, B-61, into PtK(2) cells during interphase and prometaphase. Identical results were obtained by injection of whole B-61 antiserum and of antibodies affinity-purified from the serum against a 66-kDa PtK(2) cell polypeptide. When injected into interphase cells, the antibodies arrested further development and cell division. When injected into prometaphase and metaphase cells, spindles shortened and poles moved together at a rate of 0.2-0.4 mu m/min, approximately half the rate of anaphase A chromosome movements in normally dividing PtK(2) cells. Chromosomes decondensed and cells did not reenter division. Both whole antisera and affinity-purified antibodies stained antigens diffusely localized throughout the cytoplasm in dividing and interphase cells. These results suggest that the 66-kDa antigen is a nonspindle protein that may regulate mitotic progression in PtK(2) cells.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
