Abstract
Leaf spotting was induced in soybean ( Glycine max (L.) Merrill cv. Flambeau) by each of a range of concentrations (0.0125, 0.025, 0.075, 0.10 and 0.20 M) of ethyl methanesulphonate (EMS). Significant linear relationships of EMS concentration with both the degree of leaf spotting ( P < 0.05) and the proportion of leaf mitotic aberrations ( P < 0.01) (mainly bridges and fragments at anaphase) indicate that chromosomal aberrations, and the attendant loss of genetic information, is the likely basis of leaf spotting. Preliminary findings indicate that ‘mutant’ areas of the leaf have high β-carotene content rather than depressed chlorophyll content as generally believed.
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