Abstract

SEVERAL SPECIES of the genus Chiamydomonas (Volvocales) have been studied genetically, and have proven to be interesting tools for this type of investigation (Moewus, 1940; Smith and Regnery, 1950; Lewin, 1953; Sager, 1954). In contrast, there are few studies on the nuclear cytology of these organisms. Only partial documentation is available concerning chromosome number, the pattern of mitosis and meiosis. Dangeard (1899) described the mitotic process and the chromosome number of several members of this genus. Belar (1926) published one drawing of a metaphase plate in an unidentified species, showing an acentric spindle. Kater (1929) described mitosis in C. nasuta by means of schematic drawings. No photographs were presented. Cave and Pocock (1951) studied the mitosis of 19 species of colonial Volvocales and found a range in the number of chromosomes from 5 for several Volvox species to 17 for Goniuwm pectorale. The present report deals with a study of mitosis in C. moewusii, C. eugametos, C. reinhardi and C. dysosmos. MATERIALS AND METHODS.-Cultures of C. moewusii, C. eugametos and C. dysosmos were obtained from Dr. R. A. Lewin, and cultures of C. reinhard'i from Dr. G. M. Smith and Dr. R. Sager. Cultivation.-All stock and experimental cultures were grown on Beijerinck's medium of the following composition:

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