Abstract
Mitochondrial pH (pHmito) is intimately related to mitochondrial function, and aberrant values for pHmito are linked to several disease states. We report the design, synthesis, and application of mitokyne 1-the first small molecule pHmito sensor for stimulated Raman scattering (SRS) microscopy. This ratiometric probe can determine subtle changes in pHmito in response to external stimuli and the inhibition of both the electron transport chain and ATP synthase with small molecule inhibitors. In addition, 1 was also used to monitor mitochondrial dynamics in a time-resolved manner with subcellular spatial resolution during mitophagy providing a powerful tool for dissecting the molecular and cell biology of this critical organelle.
Highlights
Mitochondrial pH is intimately related to mitochondrial function, and aberrant values for pHmito are linked to several disease states
Resting mitochondrial pH is typically alkaline as protons are pumped out of the inner mitochondrial matrix via the electron transport chain (ETC), providing a proton motive force which drives adenosine triphosphate (ATP) synthase.[22−24] ψH+ acts to regulate Ca2+ homeostasis, which, in turn, regulates elements of the tricarboxylic acid (TCA) cycle.[25−27] Alkalinization of pHmito is associated with apoptosis,[28] while acidification is associated with mitochondrial autophagy.[27,29]
We report the design, synthesis, and application of mitokyne 1 a small molecule pHmito sensor for stimulated Raman scattering (SRS) microscopy (Figure 1)
Summary
Mitochondrial pH (pHmito) is intimately related to mitochondrial function, and aberrant values for pHmito are linked to several disease states. We report the design, synthesis, and application of mitokyne 1 a small molecule pHmito sensor for SRS microscopy (Figure 1).
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
