Abstract
BackgroundMitogen activated protein kinases (MPKs) are serine/threonine protein kinases that contain characteristic T-x-Y motif in the activation loop region. MPKs are important signaling molecules involved in diverse signaling cascades that regulate plant growth, development and stress responses by conducting phosphorylation events in their target proteins. MPKs phosphorylate their target proteins at either S-P/T-P (Serine/Proline/Threonine) amino acid. To understand, if MPKs are involved in the auxin signaling cascade, we identified probable target proteins of MPKs involved in auxin signaling or transport processes.ResultsA genome-wide search of the rice genome database led us to identification of the OsAux/LAX1 gene as a potential downstream target protein of MPKs. In-silico analysis predicted that MPKs interact with OsAux/LAX1 proteins which were validated by a yeast two-hybrid assay that showed OsMPK3, OsMPK4 and OsMPK6 are physically interact with OsAux/LAX1 protein.ConclusionThe yeast two-hybrid interaction showed that MPKs are directly involved in auxin signaling events in plants. This is the first study to report direct involvement of MPKs in the auxin signaling pathway.
Highlights
Mitogen activated protein kinases (MPKs) are serine/threonine protein kinases that contain characteristic T-x-Y motif in the activation loop region
The Mitogen Activated Protein Kinase Kinase (MP2K) phosphorylate the downstream MPKs at the threonine and
It observes the steriochemical distribution of steriochemical parameters like torsion angles and covalent geometry
Summary
Mitogen activated protein kinases (MPKs) are serine/threonine protein kinases that contain characteristic T-x-Y motif in the activation loop region. MPKs are important signaling molecules involved in diverse signaling cascades that regulate plant growth, development and stress responses by conducting phosphorylation events in their target proteins. The plant mitogen activated protein kinases (MPKs) are evolutionarily conserved serine/threonine protein kinases that contain a characteristic T-x-Y motif in the activation loop region and group specific conserved docking domains in the C-terminal region [1, 2]. In the event of any environmental or cellular signaling process, plasma membrane activates MAP3Ks, which conserved serine/ threonine protein kinases that phosphorylate downstream amino acids at the S/T-X3–5-S/T motif of MP2K in the activation loop domain. We investigated whether; OsAux/LAX1 is a suitable interacting partner of MPK as it contains the S-P motif at position 88
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