Mitogen-Activated Protein Kinase MAPKKK7 from Plasmodiophora brassicae Regulates Low-Light-Dependent Nicotiana benthamiana Immunity.

Abstract

MAPKKK is the largest family of mitogen-activated protein kinase (MAPK) cascades and is known to play important roles in plant pathogen interaction by regulating fungal cell proliferation, growth, and pathogenicity. Thus far, only a few have been characterized because of the functional redundancy of MAPKKKs. In this study, it is interesting that Plasmodiophora brassicae (Pb)MAPKKK7 was clustered into the A3 subgroup of plant MAPKKKs by a phylogenetic analysis and also with the BCK1 and STE groups of fungal MAPKKKs. PbMAPKKK7 function in reactive oxygen species accumulation and cell death in Nicotiana benthamiana was characterized. Agroinfiltration with the PbMAPKKK7 mutated protein kinase domain relieved these changes. Interestingly, the induction of cell death was dependent on light intensity. Transcriptional profiling analysis demonstrated that PbMAPKKK7 was highly expressed during cortex infection stages, indicating its important role in P. brassicae infection. These functional analyses of PbMAPKKK7 build knowledge of new roles of the MAPK cascade pathway in N. benthamiana and P. brassicae interactions.