Mitochondria-rich cells in the branchial epithelium of the teleost,Oreochromis mossambicus, acclimated to various hypotonic environments

Abstract

Branchial mitochondria-rich (MR) cells were examined on the afferent side of gill filaments in tilapia (Oreochromis mossambicus) acclimated to different hypotonic environments, local fresh water (LFW), hard fresh water (HFW) and 5‰ salt water (SW). Scanning electron micrographs (SEM) identified three types of apical surfaces of the MR cells, wavy convex, shallow basin and deep hole. In spite of the different types of apical surfaces, light microscopic (LM) and transmission electron microscopic (TEM) studies suggested that these cells were MR cells. The relative abundance of these 3 types of branchial MR cells varied with external hypotonic milieus. Wavy-convexed MR cells were dominant in the gills of fish adapted to HFW, whereas shallow-basined MR cells were evident in LFW-adapted fish. In SW-adapted fish, most of the MR cells were deep holes. Experiments on adaptation to various hypotonic milieus revealed that the changes of the branchial MR cells were reversible and occurred within 24 hours following transfer. The morphological alterations of the MR cells correlated with ionic changes in different milieus, indicating that these distinct types of MR cells may play key roles for osmoregulation in hypotonic media.