Abstract
In the current study, the mitochondrial proliferationdifferentiationprocess was investigated in rat embryoduring the placentation process, straight afterorganogenesis, when there is an important oxidativemetabolism activation. For this purpose, ongestational days 11, 12 and 13 we studied themitochondrial DNA (mtDNA) content and the relativegene expression of proteins involved in mtDNAreplication (mitochondrial single strand DNA bindingprotein (mtSSB)), mtDNA transcription (mitochondrialtranscription factor A (TFAM)), as well as inmitochondrial function (cytochrome c oxidase subunitI (COXI)). The results indicated that duringplacentation important changes in mitochondrialproliferation-differentiation process take place in ratembryo. There is a great decrease in cellular mtDNAcontent and a rise in the ratio between TFAM and mtDNA accompanied by an increase in COXI geneexpression. Thus, we can conclude that on gestationalday 13 mitochondrial differentiation predominatesover mitochondrial proliferation in embryo cells.Besides, our work reveals that in a physiologicalcondition such as embryonic development the TFAMlevels change in order to regulate the transcriptionalactivity of mtDNA.
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