Mitochondrial ribosomal protein S18-2 is highly expressed in endometrial cancers along with free E2F1.

Miriam Mints,Natalia Iurchenko,Sonia Andersson,Lubov Buchynska,Muhammad Mushtaq,Daria Budnikova,Elena Kashuba,Maria C Stip,Ludmila Polischuk,Larysa Kovalevska

doi:10.18632/oncotarget.7905

Miriam Mints, Natalia Iurchenko + Show 8 more

Open Access

https://doi.org/10.18632/oncotarget.7905

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Abstract

Endometrial cancer (EC) is one of the most frequent causes of cancer death among women in developed countries. Histopathological diagnosis and imaging techniques for EC are limited, thus new prognostic markers are needed to offer patients the best treatment and follow-up.In the present paper we showed that the level of mitochondrial ribosomal protein MRPS18-2 (S18-2) increased in EC compared with the normal endometrium and hyperplasia, based on a study of 42 patient biopsies. Importantly, high expression of free E2F1 in EC correlates well with high S18-2 expression. The EC cell line HEC-1-A, which overexpresses S18-2 constitutively, showed an increased proliferation capacity in vitro and in vivo (in SCID mice). Moreover, pan-keratin, beta-catenin and E-cadherin signals are diminished in these cells, compared to the parental HEC-1-A line, in contrast to vimentin signal that is increased. This may be associated with epithelial-mesenchymal cell transition (EMT).We conclude that high expression of S18-2 and free E2F1, and low pan-keratin, beta-catenin, and E-cadherin signals might be a good set of prognostic markers for EC.

Highlights

Endometrial cancer (EC) accounts for about 6% of all cancer in females worldwide and is one of the most frequent causes of cancer death among women in developed countries
In the present paper we showed that the level of mitochondrial ribosomal protein MRPS18-2 (S18-2) increased in EC compared with the normal endometrium and hyperplasia, based on a study of 42 patient biopsies
We studied the influence of a high S18-2 expression on levels of anti-keratin, beta-catenin, E-cadherin, and N-cadherin in the EC-derived HEC1-A cell line and subline that expressed S18-2 constitutively at high levels

Summary

Introduction

Endometrial cancer (EC) accounts for about 6% of all cancer in females worldwide and is one of the most frequent causes of cancer death among women in developed countries. In cases with aggressive tumor histology, such as low differentiation or aneuploidy, a more extensive procedure is recommended, such as pelvic and paraortal lymph node evacuation and omentectomy [3]. Current imaging techniques like CT, MRI and PET have low sensitivity and specificity to locate endometrial tumors and their depth of invasion into the myometrium and cervix, or lymph node metastases. Current recommendations for surgical treatment are based on the histology of the endometrial biopsy. The current limitations of histopathological diagnosis and imaging techniques of EC require the development of biomarkers that reflect the molecular functional profile of endometrial tumors. Identification of new prognostic markers is important as it will allow us to offer the best treatment and follow-up to EC patients [5]

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