Mitochondrial Genome of Eleutheronema rhadinum With an Additional Non-coding Region and Novel Insights Into the Phylogenetics

Liqiang Zhong,Daming Li,Shengkai Tang,Xiaohui Chen,Minghua Wang

doi:10.3389/fmars.2021.746598

Liqiang Zhong, Daming Li + Show 3 more

Open Access

https://doi.org/10.3389/fmars.2021.746598

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Abstract

The complete mitochondrial genome of Eleutheronema rhadinum was determined for the first time. The E. rhadinum mitogenome was 16,718 bp in length, comprising 13 protein coding genes (PCGs), 2 ribosomal RNAs (rRNAs), 22 transfer RNAs (tRNAs), 1 control region (CR), and 1 L-strand replication origin (OL). The genome organization, and base composition were similar to other teleosts. An additional non-coding region detected in the HSL cluster, was with significant similarity to the nearby tRNA-Leu (CUN), which could be a useful marker for identifying E. rhadinum and Eleutheronema tetradactylum. Furthermore, phylogenetic analysis showed species in the family Polynemidae were well clustered together. But sister relationships between Polynemidae and Sciaenidae, Menidae plus Lactariidae were refused by present study.

Highlights

The East Asian fourfinger threadfin, Eleutheronema rhadinum, is endemic to East Asia region, widely distributed in coastal areas of China (Zhang et al, 1955; Motomura et al, 2002; Zhuang et al, 2006), Japan and northern Vietnam (Motomura et al, 2001, 2007)
The Family Polynemidae was placed in the Series Carangaria, which had a close relation with Menidae and Lactariidae (Sanciangco et al, 2016), but the Order-level was still incertae sedis (Betancur-R et al, 2017)
The variability in mitogenome length can be attributed to a new additional intergenic noncoding region between transfer RNAs (tRNAs)-Ser and tRNA-Leu (Zhang et al, 2014)

Summary

INTRODUCTION

The East Asian fourfinger threadfin, Eleutheronema rhadinum, is endemic to East Asia region, widely distributed in coastal areas of China (Zhang et al, 1955; Motomura et al, 2002; Zhuang et al, 2006), Japan and northern Vietnam (Motomura et al, 2001, 2007). Mitochondrial genome is a closed-circular molecule, ranging from 15 to 18 kb in length. It comprises a light and a heavy chain, encoding 13 protein-coding genes, 2 rRNAs, 22 tRNAs, an origin of light strand replication (OL), and a control region (Taanman, 1999). The Family Polynemidae was placed in the Series Carangaria, which had a close relation with Menidae and Lactariidae (Sanciangco et al, 2016), but the Order-level was still incertae sedis (Betancur-R et al, 2017). Its organization and characterization were fully analyzed These results would provide better understanding of the systematics of E. rhadinum, and facilitate future studies of species identification and population genetics

MATERIALS AND METHODS

RESULTS AND DISCUSSION

DATA AVAILABILITY STATEMENT