Abstract
Mitochondrial free Ca 2+ may regulate mitochondrial ATP production during cardiac exercise. Here, using laser scanning confocal microscopy of adult rabbit cardiac myocytes co-loaded with Fluo-3 to measure free Ca 2+ and tetramethylrhodamine methylester to identify mitochondria, we measured cytosolic and mitochondrial Ca 2+ transients during the contractile cycle. In resting cells, cytosolic and mitochondrial Fluo-3 signals were similar. During electrical pacing, transients of Fluo-3 fluorescence occurred in both the cytosolic and mitochondrial compartments. Both the mitochondrial and the cytosolic transients were potentiated by isoproterenol. These experiments show directly that mitochondrial free Ca 2+ rises and falls during excitation-contraction coupling in cardiac myocytes and that changes of mitochondrial Ca 2+ are kinetically component to regulate mitochondrial metabolism on a beat-to-beat basis.
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