Abstract

Abstract Variation in mitochondrial DNA (mtDNA) was assessed in a captive stock and 11 wild populations (n = 259) from throughout the native range of Desert Pupfish Cyprinodon macularius as traditionally understood. Using PCR-SSCP, 18 composite haplotypes were identified from a 333-bp segment of the mitochondrial D-loop and two segments of the ND2 gene (333 and 325 bp). Representatives of each haplotype were sequenced for the entire ND2 gene and the 337-bp segment of the D-loop. Phylogenetic analyses revealed that haplotypes form two monophyletic groups, one in the Rio Sonoyta/Quitobaquito Springs area and one in the Salton Sea/Colorado River Delta. This, with previous observations on morphology, color pattern, and geological history, supports recognition of the Rio Sonoyta/Quitobaquito populations as a separate species, the Quitobaquito Pupfish C. eremus Miller and Fuiman, from the more widespread desert pupfish C. macularius Baird and Girard. More than 70% of mtDNA diversity across all populations was a...

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