Abstract
Esophageal cancer is the eighth most common cancer globally. Esophageal adenocarcinoma (EA) and esophageal squamous-cell carcinoma (ESCC) are the two major types of esophageal cancer with poor prognosis. The mechanisms of the progression of normal esophagus to Barrett's esophagus (BE) and EA are not fully understood. Mitochondria play a central role in generating energy, apoptosis and cell proliferation. Mutations of mitochondrial DNA (mtDNA) have been identified in many diseases including cancers. Mutations of mtDNA were investigated as a part of carcinogenesis. Our objective is to study whether the 5 kb and 7.4 kb mtDNA deletions are important in the progression of normal esophagus to BE and EA. In this study, the frequency of the 5 kb and 7.4 kb deletions in mtDNA were studied in specimens ranging from normal esophageal tissue to BE and EA and also from ESCC. Seventy six paraffin-embedded tissue samples were studied. Four couple primers were used. Seventy-six tissue samples were analyzed total. The negative control and the positive control PCR product were detected in all analyzed samples. The fusion PCR products, which represent the presence of the deletions, were not detected in any of the samples. We can say that, these deletions are not associated with progression of normal esophagus to BE and EA and they do not have an important role in detecting esophagitis, BE, EA, and ESSC.
Highlights
Esophageal cancer is the eighth-most common cancer globally with an estimated 16,640 new cases and 14,500African Health SciencesBarrett’s esophagus (BE) is one of the most common premalignant lesions
Our objective is to study whether the 5 kb and 7.4 kb mitochondrial DNA (mtDNA) deletions are important in the progression of normal esophagus to BE and Esophageal adenocarcinoma (EA)
We can say that, these deletions are not associated with progression of normal esophagus to BE and EA and they do not have an important role in detecting esophagitis, BE, EA, and ESSC
Summary
Esophageal cancer is the eighth-most common cancer globally with an estimated 16,640 new cases and 14,500African Health SciencesBarrett’s esophagus (BE) is one of the most common premalignant lesions. DNA of mitochondria is circular, double stranded, closed DNA of 16569 bp in size and represents 0.1~1.0% of the total genomic DNA11 It contains 37 genes encoding 13 peptides for the oxidative phosphorylation apparatus, 7 for subunits of complex I, 1 for subunit of complex III, 3 forsubunits of complex IV, and 2 for subunits of complex V, as well as 22 tRNAs and 2 rRNAs12. Conclusion: We can say that, these deletions are not associated with progression of normal esophagus to BE and EA and they do not have an important role in detecting esophagitis, BE, EA, and ESSC. Afri Health Sci. 2019;19(1). 1671-1676. https://dx.doi.org/10.4314/ ahs. v19i1.43
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