Mitochondrial DNA D-Loop Variation among Four Sheep Breeds and Evolutionary Relationships with Other Taxa: Forensic Implication

Yasmina M Abd-Elhakim,Mayada R Farag,Amir H Abd El-Fattah,Walaa M Elhady

doi:10.17582/journal.aavs/2020/8.1.18.24

Yasmina M Abd-Elhakim, Mayada R Farag + Show 2 more

Open Access

https://doi.org/10.17582/journal.aavs/2020/8.1.18.24

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Abstract

Mitochondrial DNA D-Loop Variation among Four Sheep Breeds and Evolutionary Relationships with Other Taxa: Forensic Implication

Highlights

The analysis of nonhuman DNA has received increased consideration, in the area of forensic science, ecological investigations, and food control (Druml & Cichna-Markl, 2014)
This study directed to authenticate sheep species from other taxa to facilitate the determination of the origin of meat from commonly consumed species and commonly banned species for food control and forensic purposes through alignment of the obtained sequences with those existing in the GenBank database
To further establish the evolutionary relationship of Egyptian sheep breeds with other taxa groups, eight Mitochondrial DNA (mtDNA) control region sequences were included for analysis as follows; domestic goat (Capra hircus, KJ420477); desert goat (Capra nubiana, FJ207527); cattle (Bos taurus, AB065126); buffalo (Bubalus bubalis, AF197209); donkey (Equus asinus, KT182635); horse (Equus caballus, AF072975); dog (Canis lupus familiaris, KF574016); and pig (Sus philippensis, DQ779371)

Summary

INTRODUCTION

The analysis of nonhuman DNA has received increased consideration, in the area of forensic science, ecological investigations, and food control (Druml & Cichna-Markl, 2014). The goal of this study was to apply mtDNA control region sequence (D-Loop) analysis as a tool to evaluate the genetic diversity and phylogeography of four economically important Egyptian sheep breeds. This study directed to authenticate sheep species from other taxa to facilitate the determination of the origin of meat from commonly consumed species (cattle, buffalo, goat and pig) and commonly banned species (dog, horse and donkey) for food control and forensic purposes through alignment of the obtained sequences with those existing in the GenBank database. To further establish the evolutionary relationship of Egyptian sheep breeds with other taxa groups, eight mtDNA control region sequences were included for analysis as follows; domestic goat (Capra hircus, KJ420477); desert goat (Capra nubiana, FJ207527); cattle (Bos taurus, AB065126); buffalo (Bubalus bubalis, AF197209); donkey (Equus asinus, KT182635); horse (Equus caballus, AF072975); dog (Canis lupus familiaris, KF574016); and pig (Sus philippensis, DQ779371). Bootstrapping with 1000 replicates was used to examine the robustness of the tree

RESULTS

DISCUSSION

Conclusion

CONFLICT OF INTEREST

REFRENCES