Mitochondrial dna copy number by RT-PCR in patients with gestational diabetes mellitus in south Indian population

Abstract

Mitochondria have its own DNA called Mitochondrial DNA (mtDNA)). There are many reasons behind Mitochondrial dysfunction, may be due to point mutations, deletions, and variations in Mitochondrial DNA copy number. Pregnant women affected with GDM are associated with some alterations in mitochondria DNA copy number (mtDNA-CN). The aim of this study is to check the changes in mtDNA-CN using real-time PCR in pregnant women who are diagnosed with GDM. 25 GDM affected pregnant women and 25 healthy pregnant women were enrolled for this study. Peripheral mitochondrial copy number was checked by Real Time PCR . The result shown a mild decrease in mtDNA-CN in the pregnant women who are affected with GDM when compared to the healthy pregnant women. This reduction or variation in mitochondria DNA CN can leads to mitochondrial dysfunction.