Abstract
Ancient human DNA has various applications in molecular evolution and studies the genetic relationship between the archaic human population and the modern human population. Many ancient human remains are stored in the archeological museum and can be used for DNA sequence analysis. The current study was ever first attempt in Pakistan to use old biological specimens for molecular characterization to trace the population of Chitral district in KP Pakistan. Due to the low quantity and quality of ancient DNA, it is challenging to isolate DNA profiles from ancient human samples. A protocol was optimized for the extraction of degraded DNA from the ancient human bone's specimens. Different Bioinformatics analyses like online servers, Mitomastar and James lick, Phylogenetic Tree, and Genetic Diversity were used for the molecular characterization of the Chitrali population. Our results show that the Ancient Chitrali population has admixture with Europeans and Neolithic European populations.
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