Abstract
Tylenchidae is a widely distributed soil-inhabiting nematode family. Regardless their abundance, molecular phylogeny based on rRNA genes is problematic, and the delimitation of taxa in this group remains poorly documented and highly uncertain. Mitochondrial Cytochrome Oxidase I (COI) gene is an important barcoding gene that has been widely used species identifications and phylogenetic analyses. However, currently COI data are only available for one species in Tylenchidae. In present study, we newly obtained 27 COI sequences from 12 species and 26 sequences from rRNA genes. The results suggest that the COI gene is valid to delimitate Tylenchidae species but fails to resolve phylogenetic relationships.
Highlights
Tylenchidae is a widely distributed soil-inhabiting nematode family characterized by a weak stylet, an undifferentiated non-muscular pharyngeal corpus, and a filiform tail
RRNA genes are problematic in Tylenchidae phylogeny and the unresolved status is unlikely to be improved by intensive species sampling (Qing et al, 2017; Qing and Bert, 2019)
In this study we examined the mitochondrial Cytochrome Oxidase I gene (COI) of 12 species belong to Tylenchidae (sensu (Geraert, 2008)), the goal is to evaluate the potential of COI sequences for the identification of Tylenchidae species; and compare the resolution, sequences variability, and tree topologies obtained from one COI and two ribosomal RNA (rRNA) markers (i.e. 18S and the 28S rRNA)
Summary
Tylenchidae is a widely distributed soil-inhabiting nematode family characterized by a weak stylet, an undifferentiated non-muscular pharyngeal corpus, and a filiform tail. It comprises 412 nominal species belongs to 44 genera and estimated species number ranged from 2,000 to 10,000 species (Qing and Bert, 2019). Regardless of their abundance, the delimitation of taxa in this group remains poorly documented and highly uncertain. In this study we examined the mitochondrial Cytochrome Oxidase I gene (COI) of 12 species belong to Tylenchidae (sensu (Geraert, 2008)), the goal is to evaluate the potential of COI sequences for the identification of Tylenchidae species; and compare the resolution, sequences variability, and tree topologies obtained from one COI and two rRNA markers (i.e. 18S and the 28S rRNA)
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