Abstract
Acute ischemia was induced in the rat soleus muscle by ligation and sectioning of the abdominal aorta. Specific mitochondrial alterations were observed in the subsarcolemmal areas of ischemic muscle fibers within the first hours after the operation, at a stage when no other sign of cell damage was observed. Two types of changes were present: giant mitochondria and inclusions in the intermembrane space. The inclusions had the appearance either of accumulations of finely granular material distending the intracristal space or of plate-like structures sandwiched between the outer and the inner membrane or between two cristal membranes. The plate-like inclusions looked like single laminae located midway between mitochondrial membranes or regular rows of tubules apparently formed from helically wound filaments. The inclusions were extracted by pepsin and were unstained in preparations incubated for diaminobenzidine oxidation at pH 6.0. It is suggested that they derive from polymerization of enzymes present in the intermembrane space of muscle mitochondria, such as mitochondrial creatine kinase.
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