Abstract
Human lymphoblastoid cell line (LCL) transformed by Epstein-Barr Virus (EBV) is a unique cellular model for the study of human diseases. Although pathophysiological significance of mitochondrial calcium regulation is drawing attention, it is not known whether or not mitochondria in LCLs play a role in intracellular calcium signaling. In this study, role of mitochondria of the lymphoblastoid cell line in calcium signaling was examined. Intra-mitochondrial calcium concentration ([Ca 2+]m) was successfully measured using dihydro-Rhod-2, revealed by the decrease of fluorescence after application of carbonyl cyanide m-chlorophenylhydrazone (CCCP) and intracellular localization patterns imaged by florescent microscope. Platelet activating factor (PAF) concentration-dependently increased cytosolic calcium concentration ([Ca 2+]i), while no increase of [Ca 2+]m was observed. In contrast, 10 μM thapsigargin increased [Ca 2+]i as well as [Ca 2+]m. LCLs may be used for the study of possible pathophysiological role of mitochondrial calcium regulation in human diseases.
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