Abstract
The amount of epididymal adipose tissue hexokinase activity found to be associated with the mitochondria varied with pH due to different stabilities of the binding. Highest stability was observed when using a homogenizing buffer adjusted to pH 8.0–8.5. The binding of hexokinase to mitochondria was stabilized by glucose in vitro. No such effect of insulin was observed. No difference in activity occured during release of bound hexokinase. This indicates that the adipose tissue mitochondria do not contain any readily releasable latent hexokinase. The percentage of the adipose tissue hexokinase associated with the mitochondrial fraction was increased in carbohydrate-fed rats as compared with fasted rats. This increase was specifically related to hexokinase II. The presence of insulin (in the absence of glucose) resulted in a rapid increase in mitochondrial-bound hexokinase activity of fat pads incubated in vitro. The results suggest an interrelationship between the binding of hexokinase to mitochondria and the dietary adaptation of the enzyme.
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