Mitochondrial Barcodes of Three Malaysian Butterflies Originating from Taman Negara Endau Rompin Johor, Malaysia

Abstract

Butterflies are projected as reliable and economical biodiversity indicator. Traditionally, taxonomists identified and classified butterfly species based on highly similar and ambiguous morphological appearances which can result in problematic species identification process leading to misidentification of species. DNA barcoding has been developed for taxonomic identification of butterflies to species level. Nonetheless, this approach is hampered by the paucity of reference barcodes encompassing butterflies of all families and species in Malaysia. This study reported four novel DNA barcodes (two cytochrome oxidase subunit I (COI) and two cytochrome b, cytb) of Malaysian butterflies generated using Sanger sequencing. One barcode (DIB032 COI) supported the species identified by taxonomists whereas the other three barcodes (DIB034 COI, DIB034 cytb and DIB046/049 cytb) deduced that the butterfly species were either misidentified or unidentified up to species level due to the lack of reference barcodes in GenBank. The four novel DNA barcodes were deposited in GenBank under the accession number MT210226.1 (DIB032 COI), MT210227.1 (DIB034 COI), MT210228.1 (DIB034 cytb) and MT210229.1 (DIB046/049 cytb).