Mitochondria in the Nuclei of Rat Myocardial Cells.

Chupalav M Eldarov,Vasily A Popkov,Dmitry B Zorov,Lora E Bakeeva,Irina M Vangely,Thomas B Hildebrandt,Vladimir P Skulachev,Eugene V Sheval,Natalia G Kolosova,Susanne Holtze,Egor Y Plotnikov,Valeriya B Vays

doi:10.3390/cells9030712

Chupalav M Eldarov, Vasily A Popkov + Show 10 more

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https://doi.org/10.3390/cells9030712

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Abstract

Electron microscopic study of cardiomyocytes taken from healthy Wistar and OXYS rats and naked mole rats (Heterocephalus glaber) revealed mitochondria in nuclei that lacked part of the nuclear envelope. The direct interaction of mitochondria with nucleoplasm is shown. The statistical analysis of the occurrence of mitochondria in cardiomyocyte nuclei showed that the percentage of nuclei with mitochondria was roughly around 1%, and did not show age and species dependency. Confocal microscopy of normal rat cardiac myocytes revealed a branched mitochondrial network in the vicinity of nuclei with an organization different than that of interfibrillar mitochondria. This mitochondrial network was energetically functional because it carried the membrane potential that responded by oscillatory mode after photodynamic challenge. We suggest that the presence of functional mitochondria in the nucleus is not only a consequence of certain pathologies but rather represents a normal biological phenomenon involved in mitochondrial/nuclear interactions.

Highlights

IntroductionW. Grigg, when analyzing ultrathin sections of lymph nodes of adult mice found clustering of mitochondria around the concavities in the nuclear membrane, some lying in very close juxtaposition to the membrane [1]
These visually were identified with a variety of mitochondrial dyes, at least one of these being potential-dependent observed structures were identified with a variety of mitochondrial dyes, at least one of these being tetramethyl rhodamine methyl ester (TMRM)
Mitochondrial dye nonyl acridine apparently interacting with mitochondrial cardiolipin independently of the existence of the membrane orange (NAO), apparently interacting with mitochondrial cardiolipin independently of the existence potential (Supplementary Video S4), as well as Mitotracker Deep Red, revealed the of the membrane potential (Supplementary Video 4), as well as Mitotracker Deep Red, same peri(intra) nuclear mitochondrial network suggesting that these nuclear mitochondria are fully revealed the same peri(intra) nuclear mitochondrial network suggesting that these nuclear functional

Summary

Introduction

W. Grigg, when analyzing ultrathin sections of lymph nodes of adult mice found clustering of mitochondria around the concavities in the nuclear membrane, some lying in very close juxtaposition to the membrane [1]. Grigg, when analyzing ultrathin sections of lymph nodes of adult mice found clustering of mitochondria around the concavities in the nuclear membrane, some lying in very close juxtaposition to the membrane [1] They even suggested the presence of mitochondria inside of the nucleus but given that the quality of electron microscopic images was not perfect, this suggestion stayed hypothetical. Mori described mitochondria in nuclei of cells from four types of ascites cancer, as well as of tongue cancer, pancreatic cancer, and in regenerating hepatocytes of newts [2]

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