Abstract
Melanin synthesis is a defense mechanism that prevents skin damage, but excessive accumulation of melanin occurs in the skin in various reactions such as pigmentation, lentigines, and freckles. Although anti-melanogenic effects have been demonstrated for various naturally occurring marine products that inhibit and control tyrosinase activity, most studies have not been extended to in vivo applications. Phlorofucofuroeckol-A (PFF-A, 12.5–100 µM) isolated from Ecklonia cava has previously been shown to have tyrosinase-mitigative effects in B16F10 cells, but it has not been evaluated in an in vivo model, and its underlying mechanism for anti-melanogenic effects has not been studied. In the present study, we evaluated the safety and efficacy of PFF-A for anti-melanogenic effects in an in vivo model. We selected low doses of PFF-A (1.5–15 nM) and investigated their mitigative effects on pigmentation stimulated by α-MSH in vivo and their related-mechanism in an in vitro model. The findings suggest that low-dose PFF-A derived from E. cava suppresses pigmentation in vivo and melanogenesis in vitro. Therefore, this study presents the possibility that PFF-A could be utilized as a new anti-melanogenic agent in the cosmeceutical industries.
Highlights
IntroductionKnown as melanogenesis, is a defense mechanism that prevents ultraviolet (UV)-radiation-induced skin damage
Received: 20 January 2022Melanin synthesis, known as melanogenesis, is a defense mechanism that prevents ultraviolet (UV)-radiation-induced skin damage
We investigated the anti-melanogenic activity of low doses of PFF-A isolated from E. cava in an in vivo zebrafish model
Summary
Known as melanogenesis, is a defense mechanism that prevents ultraviolet (UV)-radiation-induced skin damage. Tyrosinase is a copper-ion enzyme that is present in a wide variety of microorganisms, plants, and animals It is a crucial enzyme in regulating the metabolic pathway of melanin formation [14]. Previous studies have reported that marine brown algae have anti-melanogenic effects through tyrosinase inhibition [19]. Low doses of PFF-A derived from E. cava were investigated for their anti-melanogenic effects, with a focus on tyrosinase inhibition activity and depigmentation in vivo and in vitro. We measured the amount of accumulated melanin after α-MSH stimulation in zebrafish skin and verified the anti-melanogenic effect and the mechanism of action of PFF-A in B16F10 cells. We demonstrated the antimelanogenic effect of PFF-A in zebrafish and expanded its potential application as an anti-melanogenic agent in the cosmetic and pharmaceutical industries
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
