Mitigation of sterigmatocystin exposure in cattle by difructose anhydride III feed supplementation and detection of urinary sterigmatocystin and serum amyloid A concentrations.

Naoya Sasazaki,Hiroshi Hasunuma,Osamu Yamato,Ayaka Miyashita,Seiich Uno,Johanna Fink-Gremmels,Masayuki Ohtani,Hiroaki Okawa,Emiko Kokushi,Mitsuhiro Takagi,Daisaku Matsumoto,Katsuki Toda,Masayasu Taniguchi

doi:10.5194/aab-64-257-2021

Abstract

We evaluated the effects of supplementing cattle feed with difructose anhydride III (DFA III) by measuring urinary sterigmatocystin (STC) concentrations using 20 Japanese Black cattle aged 9–10 months from one herd. DFA III was supplemented for 2 weeks for 10 animals, and non-treated animals served as controls. The natural STC concentration in the dietary feed was 0.06 (mixture of roughage and concentrate) at the beginning of the study (Day 0). The urine STC concentration was measured using liquid chromatography with tandem mass spectrometry 1 d prior to DFA III administration, 9 and 14 d thereafter, and 9 d following supplementation cessation, concomitant with the measurement of serum amyloid A (SAA). The number of heifers in which STC was detected in the urine was low (10 %) in the DFA III group compared to that (60 %) in the control group on Day 9. After 9 d following supplementation cessation (Day 23), STC concentrations were significantly lower () in the DFA III group than in the control group, although there was no difference in the number of heifers in which urinary STC was detected or in SAA concentrations between the two groups. Our findings demonstrate the effect of DFA III on reducing the urinary concentration of STC in Japanese Black cattle.

Highlights

The contamination of agricultural commodities with mycotoxins is a major worldwide challenge in agriculture and livestock production (Fink-Gremmels, 2008)
We previously reported the benefits of difructose anhydride III (DFA III), a unique non-digestible disaccharide present in commercial roasted chicory and manufactured from inulin through microbial fermentation (Sato et al, 2007; Teramura et al, 2015), which seems to have these properties
A total of 9 d after terminating supplementation (Day 23), STC concentration was significantly lower (P = 0.032, power = 0.741) in the DFA III group than in the control group, there was no difference in the number of heifers in which urinary STC was detected between the two groups

Summary

Introduction

The contamination of agricultural commodities with mycotoxins is a major worldwide challenge in agriculture and livestock production (Fink-Gremmels, 2008). Sterigmatocystin (STC) is generally considered an Aspergillus-derived mycotoxin but can be produced by some fungi of the genus Penicillium as well. This mycotoxin is considered the end product of a biosynthetic pathway in some fungal species, such as As-. There is no consensus on the maximum tolerable limit of STC in food or feed; the European Food Safety Authority (EFSA) classified STC as a possible human carcinogen (Group 2B) This classification was based on research data indicating that STC has carcinogenic, mutagenic, neurotoxic, immunogenic, and estrogenic effects in vitro and in vivo (EFSA Panel on Contaminants in the Food Chain, 2013; Kusunoki et al, 2011). We have assessed the efficiency of mycotoxin detoxification supplements in dietary feed to impair the intestinal adsorption of mycotoxins (Takagi et al, 2011; Hasunuma et al, 2012; Fushimi et al, 2014a)

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