Mithramycin- and triethylene melamine- induced sperm abnormalities in Lakeview hamsters.

Abstract

The sperm abnormality-inducing action of two potential mutagenic agents mithramycin and triethylene melamine (TEM), in inbred Lakeview hamsters was studied and results compared with similar investigations in mouse. Test hamsters received subacute intraperitoneal exposures ranging from 0.01 to 0.25 mg/kg body weight with either agent for 5 consecutive days. Testis weights, epididymal sperm numbers, and body weights were also monitored at weeks 1, 4, and 10 after treatment. Mithramycin-treated hamsters showed 21 times more sperm abnormalities than control (25% vs. 1.2%) whereas TEM elevated sperm abnormalities by eight fold. The frequency and type of aberrant sperm varied with dose and time, being the highest at weeks 1 or 4, rather than week 10, with either agent. Sperm number and testis weights remained depressed considerably from 4 to 10 weeks after treatment with either agent. Body weights in chemically treated hamsters remained within 28% of control for the test period. Even though our findings were in general agreement with those reported for mouse, the magnitude of the response and the stage of spermatogenic sensitivity in the chemical induction of sperm abnormalities in the two species were different.