MISER chromatography (multiple injections in a single experimental run): the chromatogram is the graph

Abstract

An experimental approach for rapid analysis and convenient interpretation of multiparallel experiments is described. Conventional approaches use a series of individual chromatographic runs to produce integrated peak area data, which are stored in individual data files, then transferred to a spreadsheet program and graphed to allow interpretation of experimental results. A simpler and more direct approach utilizes multiple injections within a single chromatographic run to produce a continuous trace of chromatograms, which can often provide a direct visual readout of experimental outcome without the need for peak integration, data transfer, or graphing. In this approach, the chromatogram itself serves as the graph whereby the outcome of the multiparallel experiment can be discerned. The utility of the technique is greatly enhanced by the use of compound-specific detection technologies such as mass spectrometry or chiroptical spectroscopy, and can benefit from experimental designs that facilitate the direct interpretation of results.