Abstract
The aim of the present study was to determine the classification error probabilities, as lean or obese, in hypercaloric diet-induced obesity, which depends on the variable used to characterize animal obesity. In addition, the misclassification probabilities in animals submitted to normocaloric diet were also evaluated. Male Wistar rats were randomly distributed into two groups: normal diet (ND; n=31; 3.5 Kcal/g) and hypercaloric diet (HD; n=31; 4.6 Kcal/g). The ND group received commercial Labina rat feed and HD animals a cycle of five hypercaloric diets for a 14-week period. The variables analysed were body weight, body composition, body weight to length ratio, Lee Index, body mass Index and misclassification probability. A 5% significance level was used. The hypercaloric pellet-diet cycle promoted increase of body weight, carcass fat, body weight to length ratio and Lee Index. The total misclassification probabilities ranged from 19.21% to 40.91%. In conclusion, the results of this experiment show that misclassification probabilities occur when dietary manipulation is used to promote obesity in animals. This misjudgement ranges from 19.49% to 40.52% in hypercaloric diet and 18.94% to 41.30% in normocaloric diet.
Highlights
Obesity is a complex multifactor disease characterized by the excessive accumulation of adipose tissue that may impair health
Rats were randomly distributed into two groups: normal diet (ND; n=31) and hypercaloric diet (HD; n=31)
No significant difference was observed in the body mass index (Table 2)
Summary
Obesity is a complex multifactor disease characterized by the excessive accumulation of adipose tissue that may impair health. Since studies on the consequences of obesity in humans have ethical limitations, experimental models of diet-induced obesity are appropriate for studying this pathology, and many studies have been developed to obtain obese animals through diet changes (Sclafani & Springer 1976; Ratting & Clark 1984; Akiyama et al 1996; Jang et al 2003; Levin et al 2003; Woods et al 2003; Dourmashkin et al 2005). Obesity in laboratory animals has been quantified by measuring visceral, epididymal and subcutaneous fat deposits (Woods et al 2003; Thibault et al 2004; Dourmashkin et al 2005), total body fat
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