Abstract
Nile tilapia is the third most cultivated fish worldwide and a novel model species for evolutionary studies. Aiming to improve productivity and contribute to the selection of traits of economic impact, biotechnological approaches have been intensively applied to species enhancement. In this sense, recent studies have focused on the multiple roles played by microRNAs (miRNAs) in the post-transcriptional regulation of protein-coding genes involved in the emergence of phenotypes with relevance for aquaculture. However, there is still a growing demand for a reference resource dedicated to integrating Nile Tilapia miRNA information, obtained from both experimental and in silico approaches, and facilitating the analysis and interpretation of RNA sequencing data. Here, we present an open repository dedicated to Nile Tilapia miRNAs: the “miRTil database”. The database stores data on 734 mature miRNAs identified in 11 distinct tissues and five key developmental stages. The database provides detailed information about miRNA structure, genomic context, predicted targets, expression profiles, and relative 5p/3p arm usage. Additionally, miRTil also includes a comprehensive pre-computed miRNA-target interaction network containing 4936 targets and 19,580 interactions.
Highlights
Nile tilapia (Oreochromis niloticus) is an economically important Teleostei species belonging to the African family of Cichlidae
This data variation was explained as differences in the experimental design [24]. These results indicate that the miRNA identification method was consistent with the other studies and that the number of Nile tilapia miRNAs annotated is within those expected for Teleostei species
For genes with an annotated stop codon, we considered, as 3’-untranslated regions (3’ UTR), the sequence extracted from the 500 nucleotides downstream of the last stop codon
Summary
Nile tilapia (Oreochromis niloticus) is an economically important Teleostei species belonging to the African family of Cichlidae. Nations [1], Nile tilapia is the third most farmed freshwater fish in the world and is especially relevant for developing countries [2,3]. Data from the plant science community have shown that economically interesting traits, such as disease resistance, biomass production, plant development, and environmental biotic or abiotic stress, can be. Cells 2020, 9, 1752 altered by manipulation of microRNAs (miRNAs) [8,9,10]. These endogenous small RNA molecules of approximately 22 nucleotides in length are post-transcriptional regulators of gene expression acting by pairing completely or partially to messenger RNAs (mRNAs), especially in the 3’-untranslated regions (3’ UTR) [11,12,13]
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