MiRNAs signature in microdissected tissues and primary cultured cells of malignant mesotelioma.

Abstract

e21031 Background: Patients diagnosed for malignant mesothelioma (MM) have poor prognosis and show poor response to conventional chemotherapy. As a consequence, there is a compelling need to identify new targets for the management of MM patients. A growing body of evidence suggests that miRNAs play crucial roles in tumorigenesis of many cancer types. However, their role in MM progression, diagnosis and prognosis is not clear and exhaustive. Methods: miRNA profiling was analyzed in a total of 7 MM compared to 2 normal mesothelium (NM) micro-dissected paraffine embedded tissue specimens and, furthermore, in 6 human cultured primary cell lines (3 MM and 3 NM). The differently expressed miRNAs in tissues and cultured cells were validated by real time PCR. Independent samples T-test was used to determine statistical significance between samples. P <0.05 was considered significant. Results: The miRNA analysis shows that 5 miRNAs are strongly de-regulated in MM compared to NM samples: 2 of them are hypo-expresse...