MiRNA expression in the cartilage of patients with osteoarthritis

Marta Kopańska,Natalia Gabło,Mateusz Trzeciak,Sławomir Snela,Dariusz Szala,Krzysztof Gargasz,Izabela Zawlik,Joanna Czech

doi:10.1186/s13018-017-0542-y

Marta Kopańska, Natalia Gabło + Show 6 more

Open Access

https://doi.org/10.1186/s13018-017-0542-y

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Journal: Journal of Orthopaedic Surgery and Research	Publication Date: Mar 28, 2017
Citations: 61	License type: open-access

Affiliation: Rzeszów University

Abstract

BackgroundOsteoarthritis (OA), the most prevalent disease of articulating joints, is a complex multifactorial disease caused by genetic, mechanical, and environmental factors. In this research, we evaluated miRNA expression in OA.MethodsForty tissue samples from 29 patients undergoing joint replacement for OA were evaluated. Tissue from two control patients undergoing hip replacement not related to OA was used as a control. Total RNA (containing miRNA species) from cartilage was isolated using a mirVana miRNA Isolation Kit. Expression of 19 miRNAs was assessed by real-time quantitative polymerase chain reaction.ResultsExpression of four miRNAs, miR-138-5p, miR-146a-5p, miR-335-5p, and miR-9-5p, was significantly upregulated in OA tissues (patients vs. control group).ConclusionsThese findings may contribute to disease prevention and the development of therapeutic targets for OA.

Highlights

Osteoarthritis (OA), the most prevalent disease of articulating joints, is a complex multifactorial disease caused by genetic, mechanical, and environmental factors
Of the 19 miRNAs, the expression of 4 was higher than 2-fold change (OA patients compared with controls, Fig. 1)
No statistically significant relationship was detected between the clinical data (Table 1) and the miRNA expression values

Summary

Introduction

Osteoarthritis (OA), the most prevalent disease of articulating joints, is a complex multifactorial disease caused by genetic, mechanical, and environmental factors. Osteoarthritis (OA) is a complex multifactorial disease caused by genetic, mechanical, and environmental factors, and it is the most prevalent disease of the articulating joints. Many environmental factors, such as hormones, diet, infections, injury, alcohol intake, and exposure to tobacco smoke, are associated with an increased risk of OA. Environmental factors induce epigenetic mechanisms that regulate genomic activity independently of changes in the DNA sequence and alter the expression of genes involved in disease development [2].

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