Abstract
BackgroundThe imbalance of expression of microRNA-802 may have a significant place in tumor progression. However, the bio-function of epithelial ovarian cancer cells remains unclear. Therefore, we setup this study to explore the pathogenesis of epithelial ovarian cancer based on microRNA-802.MethodsRT-qPCR analysis was used to measure the expression level of microRNA802 and YWHAZ in epithelial ovarian cancer. CCK-8, colony formation, flow cytometry and transwell assay were used to detect the effects of microRNA-802 on cell proliferation, apoptosis, invasion and migration. Target gene prediction and screening, luciferase reporting experiments were applied to validate the downstream target genes of microRNA-802. The effects of microRNA-802 on the expression of YWHAZ and its biological effects were measured by Western blotting and RT-qPCR.ResultsCompared with normal cell lines and tissues, the expression level of microRNA-802 was obviously down-regulated in cancer related cell lines and tissues. Overexpression of microRNA-802 could obviously inhibit the invasion and proliferation and induce apoptosis. In addition, YWHAZ was the binding target protein of miR-802 for epithelial ovarian cancer cells. YWHAZ was obviously up-regulated in human epithelial ovarian cancer cells, and YWHAZ was negatively correlated with the expression of miR-802. YWHAZ can partly eliminate the inhibitory effect caused by overexpression of miR-802 on growth and metastasis of epithelial ovarian cancer cells.ConclusionmiR-802 can regulate the occurrence and development of epithelial ovarian cancer by targeting YWHAZ.
Highlights
The imbalance of expression of microRNA-802 may have a significant place in tumor progression
Many scholars have done a lot of research on epithelial ovarian cancer, and current research focuses on gene regulation levels, including DNA methylation, group egg protein modification, and non-coding RNA, among which non-coding RNA has received unprecedented attention [8, 9]
Expression of miR-802 is down regulated in epithelial ovarian cancer cell lines and tissues First, the expression level of miR-802 gene in epithelial ovarian cancer tissues was analyzed
Summary
The imbalance of expression of microRNA-802 may have a significant place in tumor progression. MicroRNAs (miRNAs) are a class of small (19 to 25 nt), Yang et al Journal of Ovarian Research (2019) 12:100 non-coding, highly stable RNAs that regulate mRNA and protein expression [13]. They should specify that several studies have indicated that miRNAs have been involved in regulating various biological processes, such as cellular differentiation, proliferation, angiogenesis, metabolism and cancer development [14, 15]. Some studies have showed that miRNA-802 has obvious differences in the expression levels of various tumor cells and surrounding healthy tissues [19, 20]. Current research on miRNA-802 in ovarian cancer is rarely reported
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