Abstract
Background Early diagnosis of ankylosing spondylitis (AS) is yet not elucidated, with a potential diagnostic glance of microRNAs (miRNAs). Aim Study the expression profile of miRNA-451a and miRNA-125a in AS patients and their impact on disease activity and prognosis. Methods A cross-sectional study included 55 AS patients diagnosed according to modified New York criteria in 1984 with 55 matched healthy controls. History, clinical examination, and disease activity assessment with Bath ankylosing spondylitis disease activity index (BASDAI) were done. Full laboratory and radiological assessment along with expression profile of m iRNA-451a and miRNA-125a were tabulated and analyzed. Results Higher expression levels of miRNA-125a and lower of miRNA-451a in AS patients compared to controls. Furthermore, miRNA-125a expression was high in active AS patients compared to inactive patients and controls (7.0 ± 3.4 vs. 4.1 ± 2.1 vs. 2.6 ± 0.6, p < 0.001, respectively). miRNA-451a was significantly lower in active AS patients compared to inactive patients and controls (2.2 ± 1.1 vs. 4.1 ± 2.3 vs. 7.1 ± 4.5, respectively). Both miRNAs (miRNA-125a and miRNA-451a) had evident accuracy for AS diagnosis with areas under the curve (AUC) of 0.788 and 0.802, respectively. miRNA-125a had potential impact on AS activity with AUC of 0.777. Plasma levels of both miRNAs were able to distinguish AS patients with structural damage with AUCs 0.775 and 0.692, respectively. Conclusions Both miRNA-451a and miRNA-125a were found to be of great value as sensitive noninvasive diagnostic, prognostic, and disease burden biomarker of AS patients in Egypt with suggested further studies for future therapeutic implications.
Highlights
Ankylosing spondylitis (AS) is an autoimmune inflammatory disease of young adults affecting the axial skeleton and articular joints, the sacroiliac joints
Sacroiliitis and syndesmophytes are detected by plain radiography, while earlier detection could be achieved by magnetic resonance imaging (MRI) [3]
To achieve 80% power to detect this difference using an equation based on specificity = Z2 − α2 × SP × ð1 − SPÞL2 × ð1 − prevalenceÞ, with a significance level 5%, it is estimated that 110 participants would be required
Summary
Ankylosing spondylitis (AS) is an autoimmune inflammatory disease of young adults affecting the axial skeleton and articular joints, the sacroiliac joints. MiRNA-451a was significantly lower in active AS patients compared to inactive patients and controls (2:2 ± 1:1 vs 4:1 ± 2:3 vs 7:1 ± 4:5, respectively). MiRNA-125a expression was high in active AS patients compared to inactive patients and controls (7:0 ± 3:4 vs 4:1 ± 2:1 vs 2:6 ± 0:6, p < 0:001, respectively). Both miRNAs (miRNA-125a and miRNA-451a) had evident accuracy for AS diagnosis with areas under the curve (AUC) of 0.788 and 0.802, respectively. Both miRNA-451a and miRNA-125a were found to be of great value as sensitive noninvasive diagnostic, prognostic, and disease burden biomarker of AS patients in Egypt with suggested further studies for future therapeutic implications
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
