Abstract
The specific functions and clinical significance of miR-940 in endometrial carcinoma (EC) have not been studied. First, we assessed the expression of miR-940 and MRVI1 in EC tissues collected from The Cancer Genome Atlas (TCGA) database and EC cell lines. miR-940 was significantly overexpressed in EC tissues and cell lines, particularly in RL95-2 cells. Correlation analysis showed that miR-940 expression level was remarkably associated with age, grade, and death. Moreover, the overall survival (OS) rate in the miR-940 low expression group was higher, compared with miR-940 high expression group. Univariate and multivariate models demonstrated that miR-940 expression, stage, and age were predictive indicators of OS. Moreover, there was no significance of the proliferation ability among the three EC cell lines (RL95-2, ISK, and KLE). To reveal the biological roles of miR-940, we respectively transfected RL95-2 cells with miR-940 mimics, miR-940 inhibitors, and control to further investigate the cell proliferation ability, and migration as well as invasion potential of RL95-2 cells. The transfection of miR-940 mimics significantly increased the proliferation and migration/invasion ability of RL95-2 cells. MRVI1 was predicted to be a potential target of miR-940 by means of in silico analysis followed by validation using luciferase reporter assays. MRVI1 was correlated with good prognosis. Moreover, forced expression of MRVI1 in miR-940 mimic transfected cells abolished the facilitation of miR-940 on cell proliferation, migration, and invasion of RL95-2 and KLE cells. In conclusion, our study demonstrates that miR-940 might function as a reliable diagnostic and prognostic signature in EC.
Highlights
Endometrial cancer (EC) is one of the most common reproductive system malignancies for females in the world, and the incidence of this disease is rapidly increasing in recent years [1]
With the goal of clarifying the biological functions of miR-940 in EC, we investigated the expression of miR-940 in 546 EC tissue specimens and 33 normal specimens obtained from The Cancer Genome Atlas (TCGA) database
We found that the miR-940 was remarkably up-regulated in EC tissues, relative to normal samples (P
Summary
Endometrial cancer (EC) is one of the most common reproductive system malignancies for females in the world, and the incidence of this disease is rapidly increasing in recent years [1]. Of note, increasing annual incidence rate of 3.7% has been reported in China [2]. MicroRNAs (miRNAs), a class of small non-coding RNAs, function as the regulators of gene expression through targeting mRNA in a specific sequence way, and their dysregulation is a common characteristic during the development of tumor [7,8]. The abnormal expression of miRNAs has been suggested to be connected with the clinical outcome of patients with cancer [15,16,17]
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