MiR-708-5p inhibits the progression of pancreatic ductal adenocarcinoma by targeting Sirt3

Abstract

Numbers of studies have indicated that miRNA-708 plays an important role in many types of cancer. However, the role of miRNA-708 in pancreatic ductal adenocarcinoma (PDAC) has yet to be fully elucidated. The present study aimed to investigate the role of miRNA-708-5p in the proliferation, invasion and metastasis of PDAC in vitro, as well as the underlying mechanism. We found that miRNA-708-5p was upregulated in PDAC tissues and cell lines, and high miRNA-708 expression indicated poor prognosis in PDAC patients. Besides, the CCK-8 assay, colony formation assay and transwell assay results suggested that miRNA-708-5p overexpression enhanced the ability of proliferation, invasion and migration in PDAC cell lines. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR), western blotting and luciferase reporter assay demonstrated that SIRT3 was a direct target of miRNA-708-5p. Furthermore, a series of rescue experiments manifested that SIRT3 was involved in the oncogenic function of miRNA-708-5p in PDAC cells. Taken together, our study established a novel miRNA-708-5p/SIRT3 axis in the progression of pancreatic cancer and provided insight for pancreatic cancer treatment.