MiR‑589‑5p is downregulated in prostate cancer and regulates tumor cell viability and metastasis by targeting CCL‑5.

Abstract

Prostate cancer is one of the most common human malignancies, which represents a serious threat to health, and microRNAs (miRNAs/miRs) have been reported to be closely associated with the progression and development of prostate cancer. The present study aimed to investigate the expression patterns, functions and underlying mechanisms of miR‑589‑5p in prostate cancer. The results demonstrated that the expression levels of miR‑589‑5p were downregulated in prostate cancer tissues and cell lines. Overexpression of miR‑589‑5p inhibited cell viability, migration and invasion in prostate cancer cells. Subsequently, chemokine (C‑Cmotif) ligand5 (CCL‑5) was identified as a direct target gene of miR‑589‑5p, which was highly expressed at the mRNA and protein levels in prostate cancer tissues and cells. Furthermore, CCL‑5 mRNA was negatively correlated with miR‑589‑5p expression in prostate cancer tissues. Silencing CCL‑5 promoted the apoptosis, and inhibited the migration and invasion of prostate cancer cells. Taken together, these results indicated that miR‑589‑5p may act as a tumor suppressor in prostate cancer by targeting CCL‑5, thus suggesting that miR‑589‑5p may be a novel and reliable molecular marker for the diagnosis and prognosis of prostate cancer.