MiR-524 suppressed the progression of oral squamous cell carcinoma by suppressing Metadherin and NF-κB signaling pathway in OSCC cell lines

Abstract

ObjectivesThe aim of the present study was to explore the functional role of miR-524 in oral squamous cell carcinoma (OSCC) and determine its underlying mechanism. Materials and methodsTumor tissues and adjacent tissues were obtained from 55 patients with OSCC (20 females and 35 males) with a mean age of 54 years (range from 24 to 72 years). Additionally, OSCC cell lines culture was used and Reverse transcription‑quantitative PCR (RT-qPCR) was applied to measure the expression of miR-524 in OSCC tissues and cells. The protein density of Metadherin (MTDH) in OSCC tissues was detected by Immunohistochemistry (IHC) assay. MiR-524 mimic was employed to investigate the impact of miR-524 on proliferation, migration, and invasion using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and transwell assays. The dual luciferase reporter assay was utilized to investigate the interaction between MTDH and miR-524 expression. Cells transfected with miR-524 mimic and pcDNA-MTDH were subjected to western blot to investigate the role of NF-κB signaling in miR-524/MTDH axis mediated cell proliferation, migration, and invasion. ResultsMiR-524 expression was decreased significantly in OSCC tissues compared to adjacent tissues, and closely related to clinical stage, tumor size, and lymph node metastasis. Over-expression of miR-524 suppressed the proliferation, migration, and invasion of OSCC cells. Luciferase reporter assay results demonstrated that MTDH was the target gene of miR-524. Over-expression of miR-524 reduced MTDH expression and inhibited NF-κB signaling pathway. Rescue experiments revealed that over-expression of MTDH partially reversed the efficacy of miR-524 mimic on OSCC cells. ConclusionsThese results indicated that miR-524 inhibits the activation of NF-κB signaling pathway via inhibiting MTDH, resulting in the suppression of cell proliferation, migration, and invasion.