MiR-486-5p inhibits cell proliferation and invasion through repressing GAB2 in non-small cell lung cancer.

Abstract

Previous studies have reported that cell metastasis is the main reason for the high mortality of non-small cell lung cancer (NSCLC). Many miRNAs have been identified to be involved in the development of NSCLC. In this study, we explored the effect of miR-486-5p and GAB2 on cell proliferation and invasion in NSCLC. First, miR-486-5p and GAB2 expression levels were detected in NSCLC through quantitative RT-qPCR, and downregulation of miR-486-5p and upregulation of GAB2 were both identified in NSCLC. Then MTT and Transwell analysis were performed to confirm the functions of miR-486-5p and GAB2 for cell proliferation and invasion in NSCLC. Moreover, miR-486-5p overexpression was found to inhibit proliferation and invasion by suppressing GAB2 in NSCLC cells. Besides, miR-486-5p overexpression lessened GAB2 expression level in NSCLC, while miR-486-5p knockout enhanced GAB2 expression level. Additionally, miR-486-5p was identified to directly target GAB2 through dual luciferase reporter assay. The silence of GAB2 was found to inhibit proliferation and invasion of NSCLC cells. Collectively, miR-486-5p contributed to inhibiting proliferation and invasion of NSCLC cells through regulating GAB2, and miR-486-5p/GAB2 axis may provide a breakthrough for diagnosing NSCLC.