MiR-486-3p inhibits the proliferation, migration and invasion of retinoblastoma cells by targeting ECM1.

Hongwei Yang,Yu Di,Aiyuan Wang,Yonggang Huang,Dongmei Gui,Jian He,Guangrui Chai

doi:10.1042/bsr20200392

Hongwei Yang, Yu Di + Show 5 more

Open Access

https://doi.org/10.1042/bsr20200392

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Abstract

It has been reported that miR-486-3p expression is decreased in retinoblastoma (RB) tumor tissues, however, its function in RB has been less reported. The present study aimed to explore the regulatory effects of miR-486-3p on RB cells. The expression of miR-486-3p in RB tissues and cells was detected by quantitative real-time polymerase chain reaction (qRT-PCR). Cell viability, proliferation, apoptosis, migration and invasion ability were determined by cell counting kit-8 (CCK-8) kit, clone formation assay, flow cytometry, scratch assay and transwell, respectively. Targetscan 7.2 and dual-luciferase reporter were used to verify target genes for miR-486-3p. The expressions of apoptosis-related proteins and ECM1 were detected by Western blot. The miR-486-3p expression was decreased in RB tissues and cells. In RB cells, overexpression of miR-486-3p inhibited cell proliferation, migration and invasion, while promoted apoptosis. Moreover, overexpression of miR-486-3p decreased Bcl-2 expression, while increased the expressions of Bax and Cleaved Caspase-3 (C caspase-3). ECM1 was the target gene of miR-486-3p, and miR-486-3p inhibited the expression of ECM1. Furthermore, ECM1 partially reversed the inhibitory effect of miR-486-3p on the proliferation, migration and invasion of RB cells. MiR-486-3p inhibited the proliferation, migration and invasion of RB by down-regulating ECM1.

Highlights

Retinoblastoma (RB) is a common intraocular tumor in infants and young children, which seriously endangers the vision and life of children [1,2]
Compared with normal tissues and cells, the expression of miR-486-3p was significantly decreased in RB tissues and cells, which may indicate that miR-486-3p was involved in the occurrence and development of RB
The transfection of overexpressed miR-486-3p was detected by quantitative real-time polymerase chain reaction (qRT-PCR), and miR-486-3p level was highly expressed in mimic group (Figure 2A, P

Summary

Introduction

Retinoblastoma (RB) is a common intraocular tumor in infants and young children, which seriously endangers the vision and life of children [1,2]. The common treatment methods for RB are transpupillary thermal therapy, cryotherapy, chemotherapy, and ophthalmectomy, orbital exenteration and gene therapy [8,9]. Gene therapy is the treatment of a disease by manipulating a therapeutic gene or disease-related gene as a therapeutic target, which has a good prospect in the application of multiple tumor therapies [10,11]. Previous studies have demonstrated that miRNA expression may be specific to certain types of cancer and tumor-derived miRNAs may be stably detected in the plasma or serum [15,16]. Studies have found that many miRNAs are abnormally expressed in RB, such as miR-192, miR-34a and miR-376a [18,19,20,21]. MiR-125b promoted tumor growth and suppressed apoptosis by targeting DRAM2 in RB [22]. Studies have found that many miRNAs are abnormally expressed in RB, such as miR-192, miR-34a and miR-376a [18,19,20,21]. miR-125b promoted tumor growth and suppressed apoptosis by targeting DRAM2 in RB [22]. miR-188-5p promoted epithelial–mesenchymal transition via License 4.0 (CC BY)

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