MiR-450b-3p inhibited the proliferation of gastric cancer via regulating KLF7

Juan Yao,Juan Yao,Kun Zhao,Shuangshuang Chen,Cheng Liu,Rongyu Qian,Hao Zhang,Hao Zhang

doi:10.1186/s12935-020-1133-2

Abstract

BackgroundThis study aimed to investigate the clinical characteristics of miR-450b-3p in the patients of gastric cancer (GC), and further explore whether miR-450b-3p could inhibit the proliferation of GC cells via regulating KLF7.MethodsReal-time quantitative PCR (qRT-PCR) was performed to detect the expression level of miR-450b-3p in 48 GC patients of tumor tissue and paracancerous tissue specimens collected, and the associations between miR-450b-3p and the clinical characteristics of GC patients were analyzed. Meanwhile, the expression of miR-450b-3p in GC cell lines was verified using qRT-PCR. miR-450b-3p overexpression vectors was constructed in GC cell lines including AGS and BGC-823, and then CCK-8 cell proliferation assay, Plate colony formation assay and EdU assay were applied to analyze the biological function of miR-450b-3p in GC cell lines.ResultsThe results of qRT-PCR showed that the expression level of miR-450b-3p in GC tissues was lower than that in paracancerous tissues, and the difference was statistically significant. Compared with GC patients with high-miR-450b-3p expression, these GC patients with low-miR-450b-3p expression had a higher pathological stage and tumor size. Subsequently, the proliferation ability of GC cells in miR-450b-3p mimic was significantly decreased when comparing with the NC mimic. In addition, qRT-PCR indicated that the expression level of KLF7 significantly decreased after miR-450b-3p mimic. Therefore, it was demonstrated that miR-450b-3p might inhibit the malignant progression of GC via modulating KLF7. Bioinformatics analysis and dual luciferase reporter suggested miR-450b-3p was bound to KLF7. Finally, the results of the reverse experiment confirmed that overexpression of KLF7 could reverse miR-450b-3p mimic induced-inhibition of GC malignant progression.ConclusionsGenerally, miR-450b-3p significantly down-regulated in GC tissues and cell lines, and was associated with the pathological stage and tumor size of GC patients. Meanwhile, miR-450b-3p inhibited cell proliferation in GC via modulating KLF7.

Highlights

This study aimed to investigate the clinical characteristics of miR-450b-3p in the patients of gastric cancer (GC), and further explore whether miR-450b-3p could inhibit the proliferation of GC cells via regulating Krüppel-like factor 7 (KLF7)
Results miR‐450b‐3p was lowly expressed in GC tissues and cell lines The expression level of miR-450b-3p in 48 pairs of GC tumor tissue samples and corresponding paracancerous tissue ones was detected by qRT-PCR, and the results showed that miR-450b-3p expression was lower in GC tumor tissues than that in the corresponding paracancerous tissues (Fig. 1a)
MiR‐450b‐3p was correlated with pathological stage and tumor size in GC patients According to the expression level of miR-450b-3p, the above collected GC tumor tissue samples were divided into high-miR-450b-3p expression group and low-miR450b-3p expression group

Summary

Introduction

This study aimed to investigate the clinical characteristics of miR-450b-3p in the patients of gastric cancer (GC), and further explore whether miR-450b-3p could inhibit the proliferation of GC cells via regulating KLF7. Due to the lack of the early screening of GC, the diagnosis of GC is mostly confirmed in an advanced stage, and the 5-year survival rate after radical gastrectomy is only about 40% in China [3, 4]. The pathogenesis of GC is a complex multi-step and multi-stage process, involving multiple genes and environment factors [5]. The molecular targeted therapy of GC is gradually applied in clinical practice [6, 7]. How to further improve the therapeutic effect of molecular targeted drugs in advanced GC is the current problems [7]. It is necessary to find valuable diagnostic and prognostic biomarkers of GC, improving the premise of

Objectives

Methods

Results

Conclusion