Abstract
Ovarian cancer is a leading cause of gynecological cancer-related mortality. It has been reported that miR-409-3p is involved in the proliferation and migration of cancer cells. However, the role of miR-409-3p in ovarian cancer has not been well studied. The present study aimed to investigate the functional role of miR-409-3p in the pathogenesis of ovarian cancer, and its potential mechanism. It was found that the expression levels of miR-409-3p in 6 ovarian cancer tissues were upregulated. Through proliferation, migration and colony formation assays, it was revealed that overexpression of miR-409-3p inhibited the proliferation and migration of ovarian cancer cells. It was predicted from bioinformatics assays that the complementary binding sites were within miR-409-3p and Rab10. It was also demonstrated that the downregulation of the expression of Rab10 reversed the miR-409-3p downregulation-induced abnormal proliferation of ovarian cancer cells. These results suggest that miR-409-3p expression can be used as a predictive marker for the prognosis of ovarian cancer. Thus, the miR-409-3p/Rab10 axis may be a novel therapeutic target for ovarian cancer.
Highlights
Ovarian cancer is one of the common gynecological malignancies, It ranks the top three among the causes of death of female gynecological tumors in China and the fifth in the world.The causes of high mortality are difficulty in early diagnosis, limited treatment and easy recurrence, which pose a serious threat to women's health [1, 2]
These results suggest that Rab10 is ovarian cancer -related protein
Rab10 knockdown produced a negative effect on ovarian cancer cell proliferation
Summary
Ovarian cancer is one of the common gynecological malignancies, It ranks the top three among the causes of death of female gynecological tumors in China and the fifth in the world.The causes of high mortality are difficulty in early diagnosis, limited treatment and easy recurrence, which pose a serious threat to women's health [1, 2]. Studies on the pathogenesis of ovarian cancer, and the identification of new molecular markers/ targets for its early diagnosis and treatment are of considerable interest to clinicians. MicroRNAs (miRNAs) are involved in several important life processes such as growth, differentiation, cell proliferation, apoptosis, invasion, migration, and metabolism [6]. MiRNAs play a variety of roles in ovarian cancer, the miRNA research results and the diagnosis, treatment and prognosis of ovarian cancer are closely linked. It may have some theoretical value for its early diagnosis and treatment. RT-PCR was used to determine the expression level of miR-409-3p in ovarian cancer tissues and cells, and in vitro cell experiments were used to determine the effect of the miR-409-3p expression on cell proliferation and apoptosis
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