Abstract
Background: The micro-RNA miR-30b-3p has been reported to play a crucial role in several cancers. However, the biological function of miR-30b-3p in hepatocellular carcinoma (HCC) is still unknown. Methods: RT-qPCR was employed to determine the expression of miR-30b-3p in HCC tissues and cells. The MTT assay, colony formation assay, and cell migration and invasion assay were employed to evaluate the role of miR-30b-3p in HCC cells. A dual-luciferase reporter assay was employed to verify the target of miR-30b-3p. Western blotting was employed to determine the expression of key molecular signal transducers along TRIM27-PI3K/Akt axis. Results: Expression of miR-30b-3p was markedly decreased in HCC tissues and cells and positively correlated with higher overall survival. Moreover, miR-30b-3p overexpression significantly repressed cell viability, proliferation, migration, and invasion of HCC cells in vitro. Notably, we demonstrated that miR-30b-3p directly bound to the 3′-untranslated region of tripartite motif containing 27 (TRIM27) mRNA by downregulating the expression of TRIM27, which was demonstrated to be negatively correlated with miR-30b-3p expression. TRIM27 was demonstrated to have an oncogenic role in HCC cells by enhancing cell viability, proliferation, migration, and invasion. Finally, the miR-30-3p-TRIM27-PI3K/Akt axis was shown to play a crucial role in HCC cells in vitro. Conclusion: Our results indicated that miR-30-3p might act as a new biomarker for the future diagnosis and treatment HCC.
Highlights
Hepatocellular carcinoma (HCC) is still one of the deadliest and most prevalent human malignant cancers worldwide
The results showed that miR-30b-3p was downregulated markedly in HCC tumour tissues compared with matched non-carcinoma tissues (Figure 1A)
The expression of miR-30b-3p was first detected in HCC cells to verify the efficacy of miR-30b-3p mimics or anti-miR-30b-3p by Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR) assays (Figure 2A)
Summary
Hepatocellular carcinoma (HCC) is still one of the deadliest and most prevalent human malignant cancers worldwide. Increasing evidence has been uncovered that almost all diseases are related to the regulation of miRNAs, including cancers (Garzon et al, 2006; Bartels and Tsongalis, 2009; Garzon et al, 2009; Ryan et al, 2010; Chan and Tay, 2018). It has been demonstrated that miR-30b-3p was downregulated in primary prostate cancer (PCa) and metastatic castration resistant PCa and can directly inhibit androgen receptor and PCa cell proliferation (Kumar et al, 2016). The micro-RNA miR-30b-3p has been reported to play a crucial role in several cancers. The biological function of miR-30b-3p in hepatocellular carcinoma (HCC) is still unknown
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