MiR-24 reduces serum lipid levels and inhibits brain tissue cells apoptosis of rats with cerebral infarction.

Abstract

To study miR-24 effects on cerebral infarction in rats. A rat middle cerebral artery occlusion model (MCAO) was constructed. Intracerebroventricular stereotactic injection of miR-24 agomir/antagomir was performed in the rat MCAO model. According to different experiences, rats were divided into normal, sham, MCAO, miR-24 agomir and miR-24 antagomir groups. Serum TCH, HDL and TG levels were detected. Comparing the normal and sham groups, we observed decreased relative miR-24 expression (P 0.05) and increased cerebral infarction area percentage, apoptotic cells and relative caspase-3 protein expression (P 0.05) in the MCAO, miR-24 agomir and miR-24 antagomir groups. TC, TG and HDL-C levels of the MCAO and miR-24 antagomir groups were higher than those of normal and sham groups (P 0.05). Compared with the MCAO group, increased relative miR-24 expression (P 0.05) and decreased TC, TG and HDL-C levels, cerebral infarction area percentage, number of apoptotic cells and caspase-3 expression (P 0.05) were found in the miR-24 agomir group, contrasting with the observations from the miR-24 antagomir group. miR-24 reduced serum TCH, HDL and TG levels and inhibited brain tissue cell apoptosis in rats with cerebral infarction.