Abstract
BackgroundEsophageal squamous cell carcinoma (ESCC) is one of the most common and deadly forms of cancer. Despite advances in the diagnosis and treatment of this cancer, the survival rate at five years is poor. Lately, miR-22 is identified as a tumor-suppressing microRNA in many human cancers. However, the specific function of miR-22 in ESCC is unclear at this point.MethodsWe first measured miR-22 expression level in 30 paired of ESCC and matched normal tissues, ESCC cell lines by real-time quantitative RT-PCR. Invasion assay, MTT proliferation assay and wound-healing assay were performed to test the invasion and proliferation of ESCC cell after overexpression of miR-22.ResultsWe found that the expression of miR-22 in ESCC tissues and cell lines were much lower than that in normal control, respectively. The expression of miR-22 was inversely correlated with ESCC metastatic ability. Furthermore, transfection of miR-22 expression plasmid could significantly inhibit the cell proliferation, migration and invasion in Eca109 and Kyse410 ESCC cell lines.ConclusionsOur findings suggest that miR-22 act as tumor suppressor and inhibiting ESCC cell migration and invasion. The findings of this study contribute to the current understanding of the functions of miR-22 in ESCC.
Highlights
Esophageal squamous cell carcinoma (ESCC) is one of the most common and deadly forms of cancer
The expression of miR-22 is down-regulated in ESCC tissues and cell lines The expression levels of miR-22 were first evaluated in thirty paired of ESCC and normal tissues by real time Reverse transcriptionpolymerase chain reaction (RT-PCR)
We conclude that miR-22 expression is significantly downregulated in ESCC at mRNA levels in a manner negatively associated with aggressive tumor behaviors
Summary
Esophageal squamous cell carcinoma (ESCC) is one of the most common and deadly forms of cancer. Advances have been made in the treatment of ESCC, including surgery, chemotherapy, radiation or a combination of these options, the prognosis of ESCC patients remains very poor, which the overall 5-year survival rate of patient after surgery is only about 14-22% [5]. MiR-22 is a 22-nt non-coding RNA and was originally identified in HeLa cells as a tumorsuppressing miRNA. The expression and role of miR-22 in ESCC have not yet been clarified
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