MiR-214 regulate gastric cancer cell proliferation, migration and invasion by targeting PTEN

Ting-Song Yang,Xu-Dong Wang,Yi-Ling Wang,Bo Zhou,Zhen-Shun Song,Xiao-Hu Yang

doi:10.1186/1475-2867-13-68

Ting-Song Yang, Xu-Dong Wang + Show 4 more

Open Access

https://doi.org/10.1186/1475-2867-13-68

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Abstract

BackgroundMicroRNAs are a class of small non-coding RNAs that play an important role in various human tumor initiation and progression by regulating gene expression negatively. The aim of this study was to investigate the effect of miR-214 on cell proliferation, migration and invasion, as well as the functional connection between miR-214 and PTEN in gastric cancer.MethodsmiR-214 and PTEN expression was determined in gastric cancer and matched normal tissues, and human gastric cancer cell lines by quantitative real-time PCR. The roles of miR-214 in cell proliferation, migration and invasion were analyzed with anti-miR-214 transfected cells. In addition, the regulation of PTEN by miR-214 was evaluated by Western blotting and luciferase reporter assays.ResultsmiR-214 was noted to be highly overexpressed in gastric cancer tissues and cell lines using qRT-PCR. The expression level of miR-214 is significantly associated with clinical progression and poor prognosis according to the analysis of the clinicopathologic data. We also found that the miR-214 levels are inversely correlated with PTEN in tumor tissues. And PTEN expression level is also associated with metastasis and invasion of gastric cancer. In addition, knockdown of miR-214 could significantly inhibit proliferation, migration and invasion of gastric cancer cells. Moreover, we demonstrate that PTEN is regulated negatively by miR-214 through a miR-214 binding site within the 3’-UTR of PTEN at the posttranscriptional level in gastric cancer cells.ConclusionsThese findings indicated that miR-214 regulated the proliferation, migration and invasion by targeting PTEN post-transcriptionally in gastric cancer. It may be a novel potential therapeutic agent for gastric cancer.

Highlights

MicroRNAs are a class of small non-coding RNAs that play an important role in various human tumor initiation and progression by regulating gene expression negatively
The expression of miR214 is significantly associated with invasion, metastasis and TNM stage according to the clinicopathologic data from the gastric cancer patients (Table 1), and clinical relevance was confirmed by the observation that miR-214 overexpression correlated with poor prognosis (Figure 1F)
We examined the expression levels of PTEN mRNA in gastric cancer tissues and normal gastric mucosa tissues

Summary

Introduction

MicroRNAs are a class of small non-coding RNAs that play an important role in various human tumor initiation and progression by regulating gene expression negatively. The aim of this study was to investigate the effect of miR-214 on cell proliferation, migration and invasion, as well as the functional connection between miR214 and PTEN in gastric cancer. MicroRNAs, a class of small non-coding 18–25 nt in length RNAs, have been identified that it aberrantly expressed in several human malignancies, and could negatively regulate target gene expression by binding to the 3’-untranslated region (3’-UTR) of mRNAs for translational repression or degradation [4,5]. Our study results show that overexpression of miR-214 is significantly associated with metastasis and invasion and poor prognosis in gastric cancer, it could negatively regulates PTEN by binding to their 3’-UTR regions to affect gastric cancer cell proliferation, migration and invasion

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